FIGURE 3.
Reported potential Bax binding partners elute differently from gel filtration chromatography than cytosolic Bax complexes from FDM cells. Anti-Bax, anti-Mcl-1, anti-Bcl-xL, anti-14-3-3-θ, anti-Bif-1 and anti-Pin-1 Western blot analysis of gel filtration chromatography of the cytosol of FDM cells is shown. 250 μl of 1 mg cytosol in IBC-buffer was loaded onto the column and separated into 500-μl fractions. Note that whereas the elution profile of Bcl-xL, Mcl-1, and 14-3-3-θ partially overlaps with that of Bax, no such overlap is seen with Bif-1 and Pin-1 (rectangle).