The expression level and fluorescence intensity of mRFP in the fusion protein.
A, the β-galactosidase activity (green fluorescence intensity) and RFP intensity of the recombinant cells for designated times. B, Western blotting analysis of the recombinant protein at the designated times. Cell lysates from equal numbers of cells (1 × 108 cells/sample), after being induced for 2, 4, 6, 8, and 10 h, were loaded for Western blotting. The levels of the total recombinant protein were monitored by anti-His antibody. Both flow cytometric and Western blotting results were the averages of three repeated experiments.