FIGURE 2.
SUMO-BDP phosphatase activity with 32P-labeled phospho-E1b as substrate and interactions with E2b. A, pull-down assays of SUMO-BDP with lipoylated wild type, non-lipoylated K44A and lipoylated trimers. His6-tagged SUMO-BDP was incubated with a monomer excess of wild-type or mutant E2b. E2b bound to His6-tagged SUMO-BDP was extracted with Ni2+-NTA resin, eluted with imidazole, and analyzed by SDS-PAGE. B, SUMO-BDP phosphatase activity in the absence and presence of non-lipoylated K44A E2b or lipoylated wild type. Error bars, S.E.