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Enzyme kinetic parameters (kcat and Km) assayed with pNPP for wild-type and mutant SUMO-BDP carrying alanine substitutions in residues coordinated to the Mn2+ ions
SUMO-BDP phosphatase activity was assayed at 30 °C in 20 mm Tris-HCl, pH 7.5, 7 mm MnCl2 with 20 mm pNPP as a substrate.
| SUMO-BDP | kcat | Km (pNPP) | kcat/Km (pNPP) |
|---|---|---|---|
| s−1 | mm | m−1s−1 | |
| WT | 43.1 ± 1.6 | 4.4 ± 0.11 | 9,818 ± 414 |
| D109A | 0 | ||
| D127A | 0 | ||
| G128A | 44.3 ± 1.1 | 4.0 ± 0.09 | 11,075 ± 465 |
| D298A | 0 | ||
| D337A | 0 |
