Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Enzyme kinetic parameters assayed with pNPP for wild-type and mutant SUMO-BDP carrying alanine substitutions in putative catalytic residues
SUMO-BDP phosphatase activity was assayed at 30 °C in 20 mm Tris-HCl, pH 7.5, 7 mm MnCl2 with 20 mm pNPP as a substrate.
| SUMO-BDP | kcat | Km (pNPP) | kcat/Km (pNPP) |
|---|---|---|---|
| s−1 | mm | m−1s−1 | |
| WT | 43.1 ± 1.6 | 4.4 ± 0.11 | 9,818 ± 414 |
| R104A | 30.7 ± 0.9 | 4.2 ± 0.14 | 7,309 ± 389 |
| H129A | 3.07 ± 0.04 | 5.6 ± 0.08 | 548 ± 55 |
| D266A | 45.2 ± 1.3 | 3.5 ± 0.09 | 12,914 ± 524 |
