FIGURE 3.
Region between Ala713 and Cys726 in cytoplasmic domain of PC7 is essential for endocytosis of PC7. Antibody uptake experiments (30 min 4 °C, 15 min 37 °C) using the anti-FLAG antibody M2 were performed on CHO-K1 cells transfected with f-PC7 or different truncation constructs in which parts of the cytoplasmic tail of PC7 were deleted. Construct f-PC7L725A contains the entire cytoplasmic tail in which Leu725 was substituted with alanine. Double immunofluorescent staining was performed to stain cell surface-localized PC7 in green and to stain internalized PC7 in red. Scale bar = 10 μm.