FIGURE 4.

Truncation of cytoplasmic tail of PC7 beyond Ser⁷²⁷ results in increased surface localization. CHO-K1 cells transfected with the same constructs as in Fig. 3. Surface molecules were biotinylated with sulfo-NHS-LC-biotin, and the ratio of biotinylated/unbiotinylated PC7 was determined by Western blot analysis. A representative figure of this Western blot analysis is shown. Quantification of biotinylated and unbiotinylated PC7 was performed using the Image Station 440 (Kodak Digital Science). Statistical analysis was performed using the Student's t test. Values were considered to be significantly different from f-PC7 when p value < 0.05. ***, p value < 0.001. Error bars indicate S.E.