FIGURE 6.

A newly defined PLC motif is essential and sufficient for endocytosis. Chimeric proteins were constructed consisting of the luminal and transmembrane domains of Tac (CD25) and part of the cytoplasmic domain of PC7 and in which individual amino acids or groups of three amino acids were substituted with alanine to determine the exact TGN sorting motif. Antibody uptake experiments (30 min 4 °C, 15 min 37 °C) using an anti-Tac antibody were performed on CHO-K1 cells transfected with those constructs. Three amino acids (PLC) were essential for the internalization of the chimeric construct. Scale bar = 10 μm.