FIGURE 1.

Recombinant L-PGDS protein promotes the migration of microglia and astrocytes. Histidine-fused L-PGDS protein was expressed in bacteria, and separated by SDS-PAGE (A). Primary microglia cultures (1 × 10⁴ cells/upper well) (B) or primary astrocyte cultures (1 × 10⁴ cells/upper well) (C) were treated with recombinant L-PGDS protein (10–100 ng/ml) and placed in the Boyden chambers for incubation at 37 °C for 12–48 and 24–72 h, respectively, to evaluate cell migration. A representative microscopic image for each condition is shown (magnification ×100). The quantification of cell migration was done by enumerating the migrated cells as described under “Experimental Procedures.” The results are the mean ± S.D. (n = 3). *, p < 0.05 compared with the untreated control at the same time point.