FIGURE 12.

L-PGDS promotes cell migration through MARCKS. NIH3T3 fibroblast cells were transfected with control siRNA or Marcks siRNA. After 48 h, transfected cells were incubated with L-PGDS protein (100 ng/ml), and the wound healing assay was done to evaluate cell migration (A). A representative microscopic image for each condition was shown (magnification ×100) (upper). The quantification of the cell migration was done by measuring the degree of wound closure (lower). The results are the mean ± S.D. (n = 3). *, p < 0.05; between the treatments indicated. The efficiency of Marcks knockdown by siRNA was confirmed by Western blot analysis (B). α-Tubulin was detected to confirm equal loading of the samples. Similarly, after siRNA transfection, L-PGDS protein-treated cells were lysed, and p-AKT and AKT protein levels were measured by Western blot analysis (C). The results are representative of more than three independent experiments.