FIGURE 2.

The effect of recombinant L-PGDS protein on the morphology of microglia and astrocytes. The addition of recombinant L-PGDS protein (100 ng/ml) or PGD₂ (100 ng/ml) induced morphological changes in primary microglia cultures (A) and primary astrocytes (B) after 24 h. Primary microglia were stained with the peroxidase-labeled isolectin B4, followed by incubation with diaminobenzidine tetrahydrochloride (DAB) (magnification ×100 or 200). Primary astrocytes were stained with GFAP antibody (magnification ×400), followed by incubation with anti-mouse IgG-fluorescein isothiocyanate (FITC)-conjugated secondary antibody. Scale bar, 25 μm. The percentage of ramified microglia, the length of the longest process, or the number of process in each astrocyte was assessed by examining several randomly chosen microscopic fields. The results are the mean ± S.D. (n = 3). *, p < 0.05; compared with the untreated control (None).