FIGURE 3.

RNA polymerase II and STAT1 localization to the IRF1 promoter is reduced in shRNAmir-DOT1L cells. A–H, ChIP of shRNAmir non-silencing (shRNA-NS) or shRNAmir-DOT1L (shRNA-DOT1L) cells treated with IFN-γ for 30 min (right panels) or left untreated (left panels). ChIP was performed with the antibodies indicated, and qPCR, using primers spanning the IRF1 gene locus, quantified the precipitate yield reported as the percentage of input. IgG served as the negative control. p ≤ 0.05 for panels A, B, D, F, and H for solid lines with black diamonds versus dotted lines with black diamonds.