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STAT1 co-immunoprecipitates with DOT1L. A–C, whole cell extracts prepared from 2fTGH (A), shRNAmir-DOT1L (shRNA-DOT1L) (B), or shRNAmir non-silencing (shRNA-NS) (C) cells were immunoprecipitated (IP) with α-DOT1L, α-STAT1, or IgG (negative control) and then immunoblotted (IB) with the indicated antibodies. A 5% input aliquot of the extracts was included for reference.
