Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The Isa proteins are not required for the maturation of cytosolic aconitase-type proteins. isa1/2Δ and aco1Δ cells were transformed with empty vector (−) or vector overexpressing (+) yeast aconitase with (Aco1 WT) or without its mitochondrial targeting sequence (Aco1-Δsp) or the human iron regulatory protein 1 (hIRP1). Growth was in SD minimal medium, and cell extracts were prepared and analyzed for aconitase activity. Error bars, S.E. (n > 4).
