FIGURE 7.

Depletion of Iba57 results in iron accumulation on the Isa proteins. A, ⁵⁵Fe binding to Isa1 or Isa2 was determined in WT and Gal-IBA57 cells overproducing Isa1 or Isa2 as in Fig. 2. Gal-IBA57 cells were cultivated for 40 or 64 h in SD minimal medium prior to analysis. B, ⁵⁵Fe binding to the Isa proteins was determined for Gal-ISA2 cells overproducing Isa1 and isa1/2Δ cells overproducing Isa2. Gal-ISA2 cells were cultivated for either 16 or 72 h in SD medium prior to analysis. Protein levels were assessed by immunostaining of whole cell extracts. C, ⁵⁵Fe binding to the indicated proteins was determined in WT and aco1Δ cells overproducing Isa1, Isa2, or Isu1. Protein levels were assessed by immunostaining. Error bars, S.E. (n > 4).