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. 2011 Oct 4;286(48):41265–41272. doi: 10.1074/jbc.M111.300806

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Membrane translocation of EGFP-tagged PH domains in NIH 3T3 cells upon PDGF treatment. A, the time courses of subcellular localization of EGFP-tagged PDK1-PH WT and mutants in response to 50 ng/ml PDGF in NIH 3T3 cells. The arrow indicates the timing of PDGF addition. For comparison, subcellular localization of a PtdIns(3,4,5)P₃ sensor, mCerulean-tagged Btk-PH was monitored under the same conditions. B, the time course in the relative PM distribution (i.e. photon count average at PM/photon count average at (PM + cytosol)) of PDK1-PH WT, PDK1-PH-K465A, PDK1-PH-R466A/K467A, and Btk-PH calculated from Fig. 3A. Bars, 5 μm.