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. 2011 Sep 14;286(48):41368–41380. doi: 10.1074/jbc.M111.286690

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Lack of STP affects bacterial adherence to human pharyngeal cells and abrogates GAS anti-phagocytic function. The ability of the mutants and stp-complemented strains to adhere to and invade Detroit 562 human pharyngeal cells in comparison with that of the wild-type strains was determined by calculating adherence (percentage of initial inoculum) (A) and invasion (percentage of adherence) (B) indices. The multiplicity of infection was 100:1 (bacteria/cell). Error bars, S.D. of three independent experiments, each performed in six wells. C, phagocytic index of the wild-type M1SF370 and M1T1 and their corresponding mutant and complemented strains. The phagocytic index was measured as multiplication factor (M.F.), demonstrating the ability of the GAS strain to multiply in the whole human defibrinated blood as described. Error bars, S.D. of the two independent experiments performed in triplicates using blood drawn from two healthy donors.