FIGURE 1.

Primary structure and membrane topology of BNLF2a homologs. A, sequence alignment of BNLF2a from macacine herpesvirus 4 (MHV4), cercopithecine herpesvirus 12 (CHV12), pongine herpesvirus 1/3 (PHV1/3), and human herpesvirus 4 (HHV4). Identical amino acids and conserved residues are boxed in black and gray, respectively. B, transmembrane prediction of EBV BNLF2a. The TMHMM algorithm was applied. Similar results were obtained by SOSUI, HMMTOP, PredictProtein, and TMpred. A signal sequence or a corresponding cleavage site was not predicted by iPSORT, Phobius, PSORT II, SIG-Pred, SignalP 3.0, SOSUIsignal, or TargetP1.1. C, schematic presentation of EBV BNLF2a (N-terminal methionine is removed in the mature protein).