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. 2011 Oct 7;286(48):41402–41412. doi: 10.1074/jbc.M111.237784

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — BNLF2a inhibits peptide-induced conformational change of TAP. Membranes isolated from insect cells either expressing TAP1/2 with or without BNLF2a^C8-NST were incubated in the presence or absence of the peptide RRYQKSTEL (10 μm) or ICP47 (10 μm) before cross-linking with ethylene glycol bis(succinimidyl succinate) (EGS). Subsequently, samples were analyzed by SDS-PAGE (6%) and immunoblotting with TAP1- and TAP2-specific antibodies. B, BNLF2a^C8-NST does not interfere with TAP1/2 heterodimerization. Membranes isolated from Sf9 insect cells expressing either TAP1/2 or BNLF2a^C8-NST together with TAP1/2 were subjected to coimmunoprecipitation experiments (IP) using TAP1- or TAP2-specific antibodies. As a negative control, HC10-antibody was used (mock). Input (1/10 aliquot) and immune complexes (IP) were analyzed by immunoblotting. glyc, glycosylated BNLF2a; asterisk, immunoglobulin heavy chain.