FIGURE 7.

RNase H digestion assay reveals that miR-331-3p can bind both ERBB-2 WT-URE/WT-331b and ERBB-2 MT-URE/WT-331b RNAs. A, 331-3p DNA (left) or URE-Comp DNA (right) was hybridized to ERBB-2 WT-URE/WT-331b RNA (top) or ERBB-2 MT-URE/WT-331b RNA (bottom) for binding reactions with HuR, followed by RNase H digestion. RNase A (U + C) and RNase T₁ (G) digests were done in the absence of hybridizing DNA. Protein and RNA concentrations and labeling are the same as described previously. B, a schematic summarizing the digestion results obtained from A.