FIGURE 1.

CRMP1 and CRMP2 localize to mitotic microtubules. A, left, representative immunoblots for CRMP1 and CRMP2 expression in various cell lines. Right, RT-PCR CRMP products from mRNA purified from cell lines or rat brain as indicated. The black arrow points to the expected band size of about 500 bp representing CRMP5. B, representative images of endogenous CRMP2 in OLDN-93 cells. RO-3306-synchronized OLDN-93 cells were fixed 45 or 95 min after release to enrich cells with mitotic spindles or midzone MT structures. The white arrows point to regions of CRMP2 colocalization with astral MTs. Scale bar = 5 μm.