FIGURE 6.

Osteogenesis of MSC from wild-type and Hmgb2^−/− mice. A, a time-dependent reduction of Hmgb2 level in wild-type MSC (*, p < 0.05, n = 3). mono, monolayer MSC. B, after 8 days of culture under osteogenesis conditions, alizarin red S staining is much stronger in Hmgb2^−/− MSC compared with WT MSC. C, quantitative PCR shows that alkaline phosphatase (Alpl), Ibsp, Bglap, and Runx2 are significantly increased in Hmgb2^−/− MSC at day 7 (7d) upon osteogenesis induction (*, p < 0.05, n = 4). D, Western blotting shows that RUNX2 is strongly expressed in Hmgb2^−/− (KO) MSC at day 7 during osteogenesis.