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. 2011 Sep 2;286(48):41489–41498. doi: 10.1074/jbc.M111.236984

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Physical interaction of HMGB2 in the Runx2 promoter. A, interaction of in vitro-translated RUNX2 with GST-HMGB2. In vitro-translated LEF1 was used as positive control. B, ChIP assay was performed in chondrogenic ATDC5 cells using primer pairs designed to encompass the TCF/LEF element within the Runx2 promoter. LEF1, RUNX2, and HMGB2 are associated with the promoter region of the Runx2 gene but not outside this region of the Runx2 gene (Runx2, −2.5kb) and to a promoter of an unrelated gene, p19^ARF. C, summarizing schema showing functional and physical interactions between HMGB2, RUNX2, and LEF1 on the Runx2 proximal promoter containing TCF/LEF motif. HMGB2 can bind with LEF1 as well as RUNX2, and this down-regulates Runx2 promoter activity. When HMGB2 is decreased during MSC differentiation, Wnt/β-catenin-dependent activation of Runx2 promoter is enhanced, and this accelerates chondrogenesis and osteogenesis.