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. 2011 Sep 27;286(48):41510–41519. doi: 10.1074/jbc.M111.275396

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Analysis of recombinant protein reagents. A, shown are mass spectra of HPLC-purified recombinant proteins. Labels at the peak indicate the observed mass of the protein in daltons. Ther. indicates the theoretical mass of the protein in daltons. M⁻ indicates the theoretical mass of the demethylated protein. M⁻ & Ace. indicates the theoretical mass of demethylated and singly acetylated protein. B, Coomassie-stained SDS-10% PAGE analysis of the purified CT-domain proteins. Lane 1, molecular weight protein markers (Marker12, Invitrogen); lane 2, yCT purified; Lane 3, hCT purified; lane 4, yCT-H9 purified. Mass of the protein markers (in kDa) shown on the left. Each lane contained 2 μg of recombinant protein.