FIGURE 3.

Expression of ATF6(1–373) increases degradation of ATZ by proteasome. A, Hepa 1-6 cells were transiently transfected with either ATZ-pEGFP-N2 and ATF6-HA-pCGN or ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN. After 48 h, cells were incubated in the absence and in the presence of 1 μm MG132 for 8 h. SDS-PAGE of cell lysates was carried out in denaturing (panel i) and non-denaturing (panel iii) conditions, and Western blots were probed with mouse monoclonal antibodies against AAT. Panel ii, ATZ-GFP levels were measured by densitometry. Statistical significance is indicated by *** (p ≤ 0.0001) and ns (p > 0.05). Error bars = standard deviation derived from 3 independent experiments.