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. 2011 Sep 30;286(48):41656–41668. doi: 10.1074/jbc.M111.305284

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — α7 and α8 are required for membrane insertion of TP0453. A, Triton X-114 phase partitioning of WT and mutants is shown. Samples were subjected to SDS-PAGE followed by immunoblotting with monospecific antiserum directed against TP0453. aq, aqueous; det, detergent. B, incorporation of WT and mutant proteins into LUVs is shown. Lanes, bottom (BF), middle (MF) and top fractions (TF) from sucrose gradients were analyzed by SDS-PAGE and immunoblotting. C, binding curves of WT and mutants with bis-ANS show the relative changes in fluorescence intensity at 490 nm plotted against bis-ANS concentration. The inset represents a fluorescence emission spectrum of WT (1 μm) or buffer alone in the presence of 1 μm bis-ANS.