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Schematic diagram of the established procedure for quantitative cellular GSL-glycomics. Total lipids were extracted by ultrasonic homogenization in a chloroform and methanol solvent, and glycan moieties were released in the presence of a mixture of EGCase I and II (25 milliunits of each). Because of the high purification capability of glycoblotting, no further purifications were required. GSL-derived glycans were recovered as derivatives of aoWR and subjected to MALDI-TOF MS analysis.
