FIGURE 6.

MCPIP1 modulates stress-induced apoptosis. a, RAW 264.7 cells were transfected with GFP or MCPIP1-GFP plasmids. After 24 h, the transfected cells were quiescent and were stressed with 0.5 mm arsenite for 2 h. The cells were then fixed, stained with annexin V-Cy3, and observed by fluorescence microscopy. GFP images of the cells are also shown to serve as a transfected cell number control. b, quantification of Cy3-positive cells is shown. c, mouse embryonic fibroblasts from Mcpip1^−/− mice and wild-type littermates were quiescent and were stressed with 0.5 mm arsenite for 2 h. The cells were then fixed, stained with annexin V-Cy3, and analyzed by flow cytometry. d, quantification of Cy3-positive cells is shown, and data represent triplicate determinations from three independent experiments.