TABLE 2.
Fluorescence lifetime in different parts of the neurons and effect of treatment with the MARK inhibitor 39621
Fluorescence lifetime (ns) | Active growth cones | Inactive growth cones | Axon shaft | Cell body |
---|---|---|---|---|
Activity reporter | 1.897 ± 0.007 | 2.016 ± 0.017 | 2.020 ± 0.010 | 1.974 ± 0.013 |
Non-phosphorylate-able reporter (ARnp) | 2.219 ± 0.007 | 2.223 ± 0.009 | 2.199 ± 0.011 | |
After 3 h | Active growth cones | Inactive growth cones | 39621-treated growth cones | n = 15–25 |
Fluorescence lifetime (ns) | 1.910 ± 0.006 | 2.018 ± 0.010 | 2.166 ± 0.013 | |
Migration speed (μm/h) | 32.553 ± 2.515 | 4.209 ± 1.851 | 5.773 ± 1.195 |