FIGURE 2.

hSWS1 and SWSAP1 are interdependent for their stability. a–c, schematic representation of human SWS1 and SWSAP1 and their deletion mutants used in this study. b–d, mapping of the corresponding regions required for hSWS1-SWSAP1 interaction. Precipitation reactions were performed using S beads, and immunoblotting was carried out using antibodies as indicated. e and f, depletion of hSWS1 or SWSAP1 leads to the loss of both proteins in the cell. HeLa cells were transfected with control siRNA or siRNAs specifically targeting hSWS1 or SWSAP1. Lysates were analyzed by immunoblotting using antibodies recognizing hSWS1, SWSAP1, or GAPDH.