FIGURE 3.

SWSAP1 is a DNA-stimulated ATPase and preferentially binds Single-stranded DNA. a, time course for ATPase activity of SWSAP1 and hSWS1·SWSAP1 complex. SWSAP1, hSWS1·SWSAP1 complex, or SWSAP1 ATPase-inactivating mutants was incubated with or without ssDNA as described under “Experimental Procedures.” b, increasing concentrations of hSWS1, SWSAP1, hSWS1·SWSAP1 complex and SWSAP1 ATPase-inactivating mutants were incubated with 5′-biotin-labeled ssDNA or dsDNA as described under “Experimental Procedures.” Protein·DNA complexes and unbound DNA are indicated on the right.