FIGURE 5.

Titration of NHBA constructs. A, residues involved in detergent binding map on the surface of the NHBA_C1 structure as identified by chemical shift perturbation. The Cα carbons of the residues affected are indicated by yellow spheres. B, overlay of the HSQC of ¹⁵N-labeled NHBA_C2 with no heparin (blue) and with 1:10 heparin:protein molar equivalents (red). The spectra were acquired in DPC at 40 °C and 600 MHz. The titration was stopped when no additional changes were observed in two consecutive experiments.