FIGURE 5.

Confirmation of predicted miR-346 targets by qRT-PCR. A–D, mRNA levels of HLA-B (A), mRNA levels of HLA-C (B), mRNA levels of HLA-F (C), and mRNA levels of IFI6 (D). Relative mRNA levels of four selected mRNAs from supplemental Fig. 3 were tested by qRT-PCR to confirm the effects of miR-346 on the post-transcriptional regulation of these genes. Three different conditions were applied. 1) Cells were transfected with a control miRNA (C67); 2) ER stress was induced with TM (C67 TM); 3) cells were transfected with miR-346 mimic (mim 346). As suggested by the mRNA profiling following miR-346 mimic expression, both ER stress and miR-346 expression caused a significant decrease in all four mRNAs. Results are plotted as relative mRNA levels as compared with mRNA levels in cells transfected with the control miRNA, C67 (n = 4).