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In vivo bromodeoxyuridine (BrdU) labeling and flow cytometry. Four animals from each group were injected with BrdU (10 mg/kg body weight) as described elsewhere [28], prior to infection (A) and 4–5 weeks postinfection (B). Blood was collected 24 hours after BrdU inoculation. The percentages of BrdU-positive cells in CD14+ monocytes were analyzed by flow cytometry as described elsewhere [28].
