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Mapping of the AnkGAG-1D4 binding site on HIV-1 Gag CA domain. The binding activity of biotinylated AnkGAG1D4 protein was tested in ELISA towards surface-immobilized lysates of mock-infected Sf9 cells, or baculovirus-infected Sf9 cells expressing recombinant H6MA-CA, H6CA, Gagamb241, or Gagamb276 protein. The quantity of bound AnkGAG1D4 was assayed by reaction with streptavidin-HRP. Data presented are from triplicate experiments (m ± SEM).
