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. 2012 Feb 20;9:17. doi: 10.1186/1742-4690-9-17

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Copyright ©2012 Nangola et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gag-binding activity of Ank^GAG1D4 analyzed by microcalorimetry (ITC): affinity and specificity. The affinity of Ank^GAG1D4 towards its Gag substrate H6MA-CA (leftmost panel) and the affinity of Ank^A32D3 towards its αRep-A3 substrate (rightmost panel) were determined by ITC measurements of ankyrin/substrate mixtures at various protein ratios. The Gag-binding specificity was evaluated by ITC analysis of the mixture of Ank^GAG1D4 and irrelevant substrate αRep-A3 at various protein ratios (middle panel). The parameters of each binding reaction, K_dand stoichiometry (N), are shown under the corresponding panel. NA, calculation not applicable.