Table 1.

Randomization schemes used to introduce variability at specific positions of ankyrin repeats ^(a)

Repeat position	Degenerated codons	Subset of encoded amino acid
2	VDK, DMY, RAA	A, D, E, G, H, I, K, L, M, N, Q, R, S, T, V, Y
3	VDK, DMY, VAN	A, D, E, G, H, I, K, L, M, N, Q, R, S, T, V, Y
5	VDK, DMY, VAN, TGG	A, D, E, G, H, I, K, L, M, N, Q, R, S, T, V, W, Y
10	CTG, TGG, TAC, RTC	I, L, V, W, Y
13	KCK, TAC, CGY, VAR	A, E, F, I, K, L, M, Q, R, S, Y
14	KCK, VAR, AAC, SGY, YAY, NTG	A, E, G, H, K, L, M, N, Q, R, S, V, Y

^(a) For each position indicated on the leftmost column, the degenerated codons used for the microgene synthesis and the corresponding encoded amino-acids are indicated in the middle and rightmost columns, respectively. Standard letter for mixed bases refer to: N = A/T/G/C, V = A/C/G, D = A/G/T, K = G/T, M = A/C, Y = C/T, R = A/G.