Table 3.
HIV-1 integration events in control and ankyrin-expressing SupT1 cells (a)
Sequence amplified |
No ankyrin | Myr+AnkGAG1D4 | Myr0AnkGAG1D4 | Myr+AnkA32D3 |
---|---|---|---|---|
Alu-gag junctions | 28.3 ± 0.2 | 28.6 ± 0.3 | 30.5 ± 0.1 | 27.8 ± 0.2 |
GAPDH | 25.7 ± 0.4 | 26.1 ± 0.5 | 25.5 ± 0.5 | 24.5 ± 0.1 |
(a) The level of HIV-1 integration in SupT1 cells harvested at day 11 pi was evaluated by quantitative PCR amplification of host cell DNA extracts, using primers specific to Alu-gag junctions, and to cellular GAPDH gene used as the internal control. Figures shown in the Table are Cts values, mean ± SD (n = 3).