Table 4.

HIV-1 integration events in control and Raltegravir-treated SupT1 cells ^(a)

Sequence amplified	Raltegravir (nM)
	0	1	10	100
Alu-gag junctions	26.9 ± 0.2	36.1 ± 0.3	ND	ND
GAPDH	23.3 ± 0.5	23.7 ± 0.4	24.1 ± 0.3	23.9 ± 0.2

^(a) Aliquots of HIV-1-infected SupT1 cells were pretreated with Raltegravir at 0, 1, 10, and 100 nM, respectively, for 24 hr prior to HIV-1 infection (MOI 10), and the drug maintained at the indicated concentrations for 7 days. Host cell genomic DNA was extracted on day 7 pi, and the level of HIV-1 integration in SupT1 cell lines was evaluated by quantitative PCR amplification of DNA extracts, using primers specific to Alu-gag junctions and to cellular GAPDH gene as the internal control. Figures shown in the Table are Cts values (mean ± SD, n = 3). ND, not detectable (below the threshold of detection).