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. 2012 Mar 20;6(3):e1569. doi: 10.1371/journal.pntd.0001569

Figure 1. Initial comparison of plasma protein samples from Biomphalaria glabrata snail strains.

Figure 1

Plasma (cell-free hemolymph) from the NMRI (N) and BS-90 (B) strains of B. glabrata was separated into <100 kDa and >100 kDa fractions by molecular ultrafiltration, followed by separation by SDS-PAGE and staining with Coomassie brilliant blue or silver (Figure 1A). Figure 1B is a Western blot of NMRI and BS-90 plasma fractions (</>100 kDa) probed with a polyclonal antiserum to Schistosoma mansoni larval transformation products to confirm that epitope sharing in fact occurs between plasma and larval products. Note that plasma from NMRI and BS-90 snails exhibit similar patterns of protein banding (A) and anti-larval immunoreactivity (B) indicating comparable sample loading and the presence of shared epitopes. Molecular weight markers are indicated on the right.