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. 2012 Mar 20;6(3):e1575. doi: 10.1371/journal.pntd.0001575

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ZEBOVGP-specific MAbs were serially diluted, and added to an equal volume of VSVΔG/ZEBOVGP for 1 hour at 37°C before adding to a confluent 12 well tissue culture plate seeded with Vero E6 cells. After 2 days the plaques were stained and counted. The percent reduction was calculated by determining the percent reduction in plaques at each dilution in comparison to the positive control (virus with no antibody). The assay was performed in triplicate.