Figure 5. Daytime and nighttime full-length 5-HT_2C receptor mRNA expression in the hypothalamus.

A) Significantly increased expression of the full-length 5-HT_2C receptor in hypothalamus of ob/ob mice was observed in daytime. B) No change in expression of the full-length 5-HT_2C receptor was observed in animals culled in nighttime. mRNA measured using qRT-PCR relative to β-actin expression. Unpaired, two-tailed T-test; statistical significance is notated as ** p<0.01, compared to lean control; n = 7–10 per genotype.