Figure 8. 5-HT_2C receptor editing in the hypothalamus.

Expression of 5-HT_2C receptor isoforms in order of fully unedited isoform to fully edited 5-HT_2C receptor isoform. A) No change in expression of the unedited 5-HT_2C-INI isoform. B) Significantly increased expression of the edited 5-HT_2C-VNI (AB edited) isoform in hypothalamus of ob/ob mice. C) Significantly increased expression of the edited 5-HT_2C-VNV (ABD edited) isoform in hypothalamus of ob/ob mice. D) Significant increase in expression of mRNA levels of 5-HT_2C-VSV (ABCD edited) in ob/ob mice compared to control. E) Significant increase in expression of mRNA levels of 5-HT_2C-VGV (ABECD edited) isoform. All mRNA measured using qRT-PCR relative to β-actin expression and depicted as fold change compared to lean control littermates. Unpaired, two-tailed T-test; statistical significance is notated as ** p<0.01, * p<0.05 compared to lean control; n = 7–8 per genotype.