Table 2. TcUGM Reduction by NAD(P)Ha.
k red, s−1 | K d, µM | kred/Kd, µM−1 s−1 | |
NADPH | 0.60±0.006 | 98±3 | 0.0061±0.0001 |
NADH | 0.085±0.0006 | 550±10 | 0.00015±0.000002 |
Reactions were measured under anaerobic conditions at 15°C in 50 mM phosphate buffer pH 7.0.