Table 3. Steady state kinetics of TcUGM^a.

Reductant	kcat, s−1	KM, µM	kcat/KM, µM−1 s−1
Dithionite	13.4±0.3	140±10	0.093±0.006
NADPH	11.5±0.4	200±20	0.056±0.005
NADH	8.4±0.9	690±150	0.012±0.001

^aReactions were incubated at 37°C for 1 min in 25 mM Hepes, 125 mM NaCl pH 7.5, and terminated by heat denaturation at 95°C. Reactions were performed using saturating amounts of reductant (20 mM dithionite, 0.5 mM NADPH, and 2.5 mM NADH).