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. 2012 Mar 20;7(3):e33499. doi: 10.1371/journal.pone.0033499

Figure 6. Nuclear protein extracts from human BM HPCs have higher NHEJ activity than those from HSCs.

Figure 6

A. qPCR analysis of the NHEJ activity in nuclear proteins extracted from different numbers of human BM CD34+ cells. The data are presented as mean ± SD of arbitrary units (n = 3). The symbols of * and # represent p<0.05 vs. nuclear proteins from 1,000 and 5,000 cells, respectively. B. A representative gating strategy to sort human HSCs (CD34+CD38− cells) and HPCs (CD34+CD38+ cells) from human BM CD34+ cells. C. NHEJ activity in nuclear proteins extracted from the same number (6,600) of human HSCs and HPCs. The data are presented as mean ± SD of arbitrary units (n = 3 independent experiments). D. Analysis of the expression of ATM, DNA-PKcs, KU70, KU80, Ligase 4, and XRCC4 mRNA in human BM HSCs and HPCs by qRT-PCR. The data are presented as ratios between HPCs and HSCs (n = 3 independent experiments). **p<0.01 and ***p<0.001 vs. HSCs.