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. 2012 Mar 20;7(3):e33498. doi: 10.1371/journal.pone.0033498

Figure 1. THO complex components Hpr1p and Tho2p affect telomere length.

Figure 1

(A) Telomere lengthening in hpr1 and tho2 strains. Yeast DNA was isolated from the YPH499 yeast strain with indicated mutations, digested with XhoI, separated on a 1% agarose gel and analyzed by Southern blotting. The blots were hybridized with probes prepared using the 600-bp sequence from the Y′ element 3′ end. The position of the Y′ telomere is indicated. (B) Temperature-sensitive growth of THO complex mutants. Aliquots of ten-fold serial dilutions of yeast cells were spotted on YEPD plates and incubated at 30 or 37°C for three days. (C) Telomerase is required for the telomere lengthening effects in hpr1 and tho2 cells. Yeast cells carrying the indicated mutations were freshly sporulated and grown on YEPD plates at 30°C after 1 or 2 restreaks. Total yeast DNA was then isolated and analyzed by Southern blotting. (D) Recombination is not required for telomere lengthening in tho2 cells. Total yeast DNA from the indicated strains was isolated and analyzed by Southern blotting.