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. 2012 Mar 20;7(3):e33498. doi: 10.1371/journal.pone.0033498

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Yu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Yeast DNA from the indicated mutations was isolated and analyzed by Southern blotting assays using the Y′ element probe. (B) The decreased Tel1p level does not contribute to telomere lengthening in tho2 and hpr1 cells. Total mRNA from the indicated strains was isolated and analyzed for GCN1 and TEL1 RNA levels using real time RT-PCR (left panel). Results were presented as relative levels normalized to the wild-type expression level. The bars were standard deviations determined from three independent experiments. Yeast DNA from indicated strains was analyzed for telomere length (right panel). (C) Overexpressing Rif1p suppresses the telomere lengthening in tho2 and hpr1 cells. Wild-type, tho2, or hpr1 yeast cells carrying plasmids pRS426 or pRS426-RIF1 (o/e RIF1) were cultured at 30°C. Telomere lengths of these cells were then analyzed using Southern blotting assays (top panel). Immunoblotting analysis of the Rif1p level was also performed (bottom panel).