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. 2012 Mar 20;7(3):e33498. doi: 10.1371/journal.pone.0033498

Figure 5. Overexpressing SUB2 cannot restore the Rif1p level and telomere length in tho2 and hpr1 cells.

Figure 5

(A) Overexpressing SUB2 suppressed the growth defect of tho2 or hpr1 cells. Strains of the indicated genotypes derived from THO2/tho2, or HPR1/hpr1 diploids carrying SUB2 or sub2-5 overexpressing plasmids were grown on YC plates at 30° or 37°C. (B) SUB2 or sub2-5 overexpression did not restore the Rif1p level in tho2 or hpr1 cells. Immunoblotting assays were carried out as described. (C) SUB2 overexpression did not restore the RIF1 level in tho2 or hpr1 cells. Total mRNA from the indicated strains was isolated and analyzed for the RIF1 RNA level using real time RT-PCR. Results were presented as relative levels normalized to the wild-type expression level. The bars were standard deviations calculated using data from three independent experiments. (D) SUB2 or sub2-5 overexpression did not restore the telomere length in tho2 or hpr1 cells. Telomere length analyses were performed as previously described.