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. 2012 Mar 20;7(3):e33270. doi: 10.1371/journal.pone.0033270

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He et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The Macroautophagy gene MoATG1 is required for nuclear degeneration. Upper panel: time course live cell images showing nuclear division and nuclear degeneration during appressorium development in a M. oryzae ΔMoatg1 mutant. ΔMoatg1 conidia expressing H1:RFP were examined by epifluorescence microscopy at indicated time points. Lower panel: time series of bar charts showing the percentage of spores in ΔMoatg1 containing 0 to 4 nuclei (mean ± SD, n>100, triple replications). (B) The Macroautophagy core gene MoATG4 is required for nuclear degeneration. Upper panel: time course live cell images showing nuclear division and nuclear degeneration during appressorium development in M. oryzae ΔMoatg4 mutant. ΔMoatg4 conidia expressing H1:RFP were examined by epifluorescence microscopy at indicated time points during appressorium development. Lower panel: time series of bar charts showing the percentage of spores in ΔMoatg4 containing 0 to 4 nuclei (mean±SD, n>100, triple replications). Scale bar = 10 µm.